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外科研究与新技术（中英文）

外科研究与新技术（中英文） >

2013 , Vol. 2 >Issue 2: 73 - 86

DOI: https://doi.org/10.3969/j.issn.2095-378X.2013.01.001

论著

生成拟态和不良预后的意义

  • 孙伟 ,
  • 范跃祖 ,
  • 张文忠 ,
  • 葛春燕
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  • 同济大学附属同济医院外科，上海200065
孙伟(1974-)，男，主治医师，博士，研究肝胆外科。

网络出版日期: 2013-02-25

基金资助

国家自然科学基金资助项目(30672073)

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MMP-2 and MT1-MMP contribute to vasculogenic mimicry and poor prognosis in human primary gallbladder carcinomas

  • SUN Wei ,
  • FAN Yue-Zu ,
  • ZHANG Wen-Zhong ,
  • GE Chun-Yan
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  • Department of Surgery,Tongji Hospital,Tongji University School of Medicine,Shanghai 200065,China

Online published: 2013-02-25

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摘要

目的 探讨MMP-1，- 2，- 9，- 14表达与胆囊癌患者的预后和有/无血管生成拟态的相关性。方法 免疫组化染色(IHC)计算机图像定量分析、高/低免疫反应性截点得分选择和ROC曲线被用来分析基质金属蛋白酶(MMP，2，9，和14)在89例胆囊癌(GBC)表达及其与血管生成拟态(VM)和临床病理特征、预后的相关性。结果 MMP-2与MMP-14(MT1-MMP)表达明显与胆囊癌VM相关(P均＜0.01)，与Nevin分期、分化和无VM组肝转移程度相关(P均＜0.05);此外，MMP-2表达也与浆膜浸润和VM组肝转移相关(P均＜0.05)。在胆囊癌VM患者中，MMP-2表达与临床病理参数如中度分化、Nevin分期(S3-S5)、肝转移、浆膜浸润和淋巴结转移相关(P＜0.05)，MMP-14表达与分化程度(G2和G3)、Nevin分期、淋巴结转移、浆膜侵犯、阴性肝转移和浆膜浸润相关(P均＜0.05)。多变量分析显示，MMP-2与MMP-14为胆囊癌VM阴性或阳性患者总生存和不良预后的独立因素(P均＜0.05)。结论 MMP-2、MMP-14是有/无VM胆囊癌患者的独立的预后因素，在VM形成和GBC进展中可能起重要作用。

关键词： 血管生成拟态; 基质金属蛋白酶; 胆囊肿瘤; 预后

本文引用格式

孙伟 , 范跃祖 , 张文忠 , 葛春燕 . 生成拟态和不良预后的意义[J]. 外科研究与新技术（中英文）, 2013 , 2(2) : 73 -86 . DOI: 10.3969/j.issn.2095-378X.2013.01.001

Abstract

Objective To investigate MMP-1,-2,-9,-14 expressions and their correlation with prognosis of the gallbladder carcinoma patients with/without vasculogenic mimicry.Methods Immunohistochemistry (IHC) computer-assisted quantitative image analysis and receiver operating characteristic curve were used to analyze expression of MMPs (MMP-1,-2,-9,and -14) in 89 gallbladder carcinoma (GBC) samples,IHC quantitative analysis and selection of the cut-off score for high or low IHC reactivity.Correlation of MMPs expression with vasculogenic mimicry (VM),clinicopathologic features and prognosis of GBC patients with VM and non-VM was evaluated simultaneously.Reaults Over-expression of MMP-2 and MMP-14 was significantly associated with VM in GBC (both P<0.01).Moreover,the expression of MMP-2 and MMP-14 was correlated with Nevin stage,degree of differentiation and liver metastasis in non-VM group (both P<0.05).In addition,MMP-2 expression was also associated with serosal invasion and liver metastasis in VM group (both P<0.05).Over-expression of MMP-2 in GBC patients was associated with VM in the same subset of clinicopathologic parameters,including moderate degree of differentiation,Nevin stage (S3-S5),positive liver metastasis,serosal invasion and lymph node metastasis (both P<0.05).Moreover,over-expression of MMP-14 in GBC patients was correlated with VM in the same subset of clinicopathologic parameters,including degree of differentiation (G2 and G3),Nevin stage,positive lymph node metastasis and serosal invasion,negative liver metastasis and serosal invasion(both P<0.05).Multivariate analysis showed that MMP-2 and MMP-14 were independent factors for the overall survival (OS) rate and associated with poor prognosis either in VM negative or positive group (both P<0.05).Conclusion MMP-2 and MMP-14 were independent factors,associated with prognosis of the GBC patients with/without VM,might play significant role in VM formation and progression of GBC.

Key words： Vasculogenic mimicry; Matrix metalloproteinases; Matrix metalloproteinase 2; Matrix metalloproteinase 14; Gallbladder neoplasm; Prognosis

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