目的 构建Fn14-shRNA慢病毒载体，以进一步进行动物实验，研究Fn14在骨肉瘤中的表达和作用。方法 使用RNA干扰技术，构建Fn14干扰载体、病毒包装、感染细胞，并进行鉴定。结果 Fn14-shRNA干扰质粒单酶切验证阳性，测序结果与设计完全一致，干扰组Fn14在稳转细胞株中表达下调。 结论 成功构建了Fn14-shRNA慢病毒载体，为进一步从分子水平探讨Fn14在骨肉瘤中的生物学作用奠定了基础。
Objective To construct a Fn14-shRNA lentiviral vector to further study the expression and biological significance of Fn14 in osteosarcoma with animal experiment. Methods Constructing Fn14-shRNA vector by RNA interference technique, viral packaging, co-transfecting cells and indentification. Results Single enzyme detection was positive, sequencing result was consistent with design, Fn14 was downregulated in stably transfected cell lines. Conclusion The Fn14-sh RNA lentivirus recombinant vectors were successfully constructed which laid the foundation for further study of the biological significance of Fn14 in osteosarcoma.
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