目的 观察护肝解纤汤对肝纤维化过程中相关细胞因子结缔组织生长因子(CTGF)、转化生长因子(TGF-β1)、血小板衍生生长因子(PDGF)的影响及其可能机制。方法 以四氯化碳腹腔注射8周建立大鼠肝纤维化模型。实验分对照组、模型组、小柴胡汤组和护肝解纤汤组。护肝解纤汤组于大鼠肝纤维化模型鼠第9周起分别予高、中、低剂量护肝解纤汤(处方中姜黄分别为低剂量组6 g,中剂量组9 g,高剂量组15 g,其他成分一致)。免疫组化染色观察肝组织细胞因子CTGF、TGF-β1、PDGF在各组中表达。结果 与模型组比较,高、中剂量护肝解纤汤组肝组织CTGF、PDGF表达显著减少(P<0.05);TGF-β1表达只有高剂量护肝解纤汤组显著降低(P<0.05)。结论 护肝解纤汤组相关促纤维化细胞因子CTGF、TGF-β1、PDGF表达减少,可能是其影响肝纤维化的重要机制。

Objective To observe the effect and possible mechanism of Huganjiexian Decoction on liver fibrosis-related cytokines CTGF,TGF-β 1,and PDGF.Methods Liver fibrosisr at models wereestablished by intraperitoneal injection of carbon tetrachloride for eight weeks.The rats were divided into control group,model group,Minor Bupleurum Decoction group,and Huganjiexian Decoction group (administered with high,middle,and low doses of turmeric containing 15,9 and 6 g respectively,from week 9-12).Immunohistochemical staining was used to detect the expression of CTGF,TGF-β1,and PDGF in liver tissues.Results The expression levels of CTGF and PDGF in liver tissues in the high and middle dose groups significantly decreased compared to the model group(P<0.05),while the expression level of TGF-β1 decreased significantly only in the high dose group (P<0.05).Conclusion Huganjiexian Decoction could downregulate the expressions of fibrosis-promoting cytokines CTGF,TGF-β1,and PDGF,which might be one of the important mechanism protective effects of against liver fibrosis.