目的 为研究依立雄胺治疗前列腺癌的可能性及可能机制, 探讨依立雄胺体外对人激素非依赖型前列腺癌细胞( DU-145) 生长的作用。方法 用2.5, 5, 10, 20和40 μM的依立雄胺作用DU-145细胞3天后, 用普通倒置显微镜进行细胞形态学观察; 用黄酰罗丹明B法(SRB法)评价依立雄胺对前列腺癌细胞株DU-145的体外增殖抑制作用绘制的生长曲线; 应用流式细胞仪评价依立雄胺对对前列腺癌细胞株DU-145的周期阻滞作用。结果 40 μM和20 μM的依立雄胺作用DU-145细胞3 d 后, 可使其明显的皱缩凋亡;依立雄胺对DU-145呈现明显的浓度依赖的体外增殖抑制作用;通过流式细胞仪分析发现, 依立雄胺对DU-145呈现明显的浓度依赖的周期阻滞作用。
Objective To study the possibility and potential mechanisms of epristeride treatment of prostate cancer,and to investigate the effect of epristeride on the growth of DU-145 cells in vitro.Method We used different concentration of epristeride (2.5,5,10,20,40mM) to interfere DU145 cells and used an inverted microscope to observe the cell morphology.We used Sulforhodamine B(SRB) to evaluate the growth curve characterized by the inhibition of in vitro proliferation of DU145 cells caused by epristeride.We applied flow cytometry to evaluate cell cycle arrest of DU145 induced by epristeride.Results An obvious cell shrinkage and apoptosis was observed three days after the interference of epristeride with the concentration of 40mM and 20mM.Epristeride shows a conspicuously concentration-dependent inhibition of in vitro proliferation of DU145 cells.Through flow cytometry,we found that epristeride demonstrated a highly Concentration dependent effect of causing cell cycle arrest in DU145 cell line.
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