目的 上海市中医医院作为上海市的三甲医院为积极、快速执行国务院下达的指令,要求医院具备二级以上实验室,具备核酸检测的能力,进行核酸检测设备的疫情紧急采购。方法 通过了解核酸检测原理并对核酸检测仪性能,医院现有场地情况和临床科室要求进行比较,从多个预设方案中确定一个最适合我院具体情况的方案。结果 将采购全自动核酸提取仪确定为最优方案,并需额外采购5台气溶胶吸附器,放置在采样点和PCR实验室。最终在2020年6月10日完成全部工作。结论 只有了解核酸检测原理,通过场地合理布局,产品性能比较,设备及时采购,才能确保新冠病毒检测工作的顺利实现。
Objective Shanghai Traditional Chinese Medicine Hospital, as a tertiary class-A hospital in Shanghai, should be equipped with a P2 laboratory and above capable of nucleic acid testing and urgent procurement of nucleic acid testing equipment in case of epidemic. Methods After investigating the fundamentals of nucleic acid testing and comparing the performance of nucleic acid detectors, available space of the hospital, and the requirements of clinical departments, a plan that would best address the hospital needs was chosen. Results Purchasing an automatic nucleic acid extractor was selected as the best solution, as well as five aerosol adsorbers. They were placed in sampling points and PCR laboratories. The procurement was completed by June 10,2020. Conclusion The coronavirus detection can only be accomplished after understanding the fundamentals of nucleic acid testing, appropriate space layout,product performance comparison, and timely procurement of equipments.
[1] 《新型冠状病毒肺炎诊疗方案(试行第6版)》解读[J].中国医刊,2020,55(3):347-348.
[2] 黄子通,梁子敬,宋凤卿,赖荣德.广东省抗击新型冠状病毒肺炎急诊科防控工作指引(第1版)[J].岭南急诊医学杂志,2020,25(1):1-4.
[3] 瞿慧. 新生仔猪脐带血PRRSV、PRV、PCV2检测在养猪业上的应用研究[D].上海交通大学,2016.
[4] 罗英. 磁珠法核酸自动提取仪在分子生物学领域的应用[J].蚕学通讯,2013,33(2):22-28.
[5] 黄长武,单幼兰,唐彦,谭俊杰,张晓清.一种新型肠道病毒核酸检测试剂的初步评价[J].检验医学与临床,2015,12(7):907-909.