目的 制备氧化再生纤维素/壳聚糖复合物（ORC-CTS）止血海绵并研究其性能。方法 制备并表征ORC-CTS止血海绵。通过体内止血实验、炎症反应及毒性分析,比较ORC-CTS与壳聚糖、速即纱的体内止血性能及效果。结果 ORC-CTS组的出血量明显少于空白对照组、壳聚糖组、速即纱组（均P<0.05）,止血时间亦明显短于空白对照组、壳聚糖组、速即纱组（均P<0.05）;炎症程度依次为对照组<ORC-CTS<壳聚糖<速即纱组;ORC-CTS组血清TNF-α、异前列腺素和vWF与对照组无差异。结论 ORC-CTS能显著减少出血量,缩短出血时间。生物体内炎症反应轻,有良好的生物相容性,在生物体内几乎无毒,有望成为一种理想的新型止血材料。
Objective To prepare oxidized regenerated cellulose/chitosan （ORC-CTS） hemostatic sponges, and study its properties. Methods ORC-CTS hemostatic sponges were prepared and characterized. The hemostatic properties and prognostic effects of ORC-CTS, chitosan, and surgicel were compared by hemostasis test, inflammation test, and toxicity test in vivo. Results The blood loss in the ORC-CTS group was less than that in the control group, the chitosan group, and the surgicel group （all P<0.05）; the hemostasis time in the ORC-CTS group was shorter than that in the control group, the chitosan group, and the surgicel group （all P<0.05）. The degree of inflammation ranked as control group<ORC-CTS group<chitosan group<surgicel group. No significant differences in serum tumor necrosis factor （TNF-α）, isoprostanes, and von Willebrand Factor （vWF） were found between the ORC-CTS group and the control group. Conclusion ORC-CTS hemostatic sponges can reduce blood loss, shorten bleeding time, stimulate mild inflammatory reaction, show good biocompatibility and little toxicity, thus may be an ideal new hemostatic material.
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