Fn14-shRNA慢病毒载体的制备

外科研究与新技术 ›› 2016, Vol. 5 ›› Issue (2): 96-101.

Fn14-shRNA慢病毒载体的制备

于涛¹,杨云峰²,李兵¹,陈凯¹,朱辉¹,张明珠¹,赵有光¹,俞光荣¹

1. 上海市同济医院，同济大学附属同济医院
2. 上海市同济医院同康楼

收稿日期:2016-01-18 修回日期:2016-01-19 出版日期:2016-06-28 发布日期:2016-09-13
通讯作者: 俞光荣 E-mail:yuguangrong2002@126.com

Construction and identification of Fn14-shRNA lentiviral vector

Received:2016-01-18 Revised:2016-01-19 Online:2016-06-28 Published:2016-09-13

摘要/Abstract

摘要： 目的构建Fn14-shRNA慢病毒载体，以进一步进行动物实验，研究Fn14在骨肉瘤中的表达和作用。方法使用RNA干扰技术，构建Fn14干扰载体、病毒包装、感染细胞，并进行鉴定。结果 Fn14-shRNA干扰质粒单酶切验证阳性，测序结果与设计完全一致，干扰组Fn14在稳转细胞株中表达下调。结论成功构建了Fn14-shRNA慢病毒载体，为进一步从分子水平探讨Fn14在骨肉瘤中的生物学作用奠定了基础。

关键词: 骨肉瘤, 细胞表面受体成纤维细胞生长因子诱导14（Fn14）, RNA干扰, 慢病毒

Abstract: Objective To construct a Fn14-shRNA lentiviral vector to further study the expression and biological significance of Fn14 in osteosarcoma with animal experiment. Methods Constructing Fn14-shRNA vector by RNA interference technique, viral packaging, co-transfecting cells and indentification. Results Single enzyme detection was positive, sequencing result was consistent with design, Fn14 was downregulated in stably transfected cell lines. Conclusion The Fn14-sh RNA lentivirus recombinant vectors were successfully constructed which laid the foundation for further study of the biological significance of Fn14 in osteosarcoma.

Key words: osteosarcoma, Fibroblast growth factor-inducible14 (Fn14), RNA intervention, lentiviral

于涛杨云峰李兵陈凯朱辉张明珠赵有光俞光荣. Fn14-shRNA慢病毒载体的制备[J]. 外科研究与新技术, 2016, 5(2): 96-101.

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