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Original articles

Vasculogenic mimicry,HIF-1α and VEGF expression in gallbladder carcinoma:association with clinical outcomes and prognostic implications

  • Sun Wei ,
  • Fan Yue-Zu ,
  • Zhang Wen-Zhong ,
  • Ge Chun-Yan
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  • Department of Surgery,Tongji Hospital,Tongji University School of Medicine,Shanghai 200065,China

Online published: 2012-01-25

Abstract

Purpose To explore whether VM exists in human gallbladder carcinoma (GBC) and VM’ correlation with HIF-1α,VEGF and prognosis of GBC patients.Methods HE and CD31-PAS staining were used to observe VM in GBC.Immunohistochemistry (IHC),computer-assisted image and receiver operating characteristic curve were used to analyze expression of HIF-1α and VEGF in GBC,IHC quantitative analysis and selection of the cut-off score for high or low IHC reactivity.Correlation of HIF-1α and VEGF expression with clinicopathologic features and prognosis of GBC patients with VM and non-VM was evaluated simultaneously.Results VM was observed in GBC (21.35%) and associated with adenocarcinoma and liver metastasis (both P<0.01).Over-expression of HIF-1α and reduced-expression of VEGF were observed in GBC patients with VM (both P<0.01).HIF-1α was correlated with serosal invasion,degree of differentiation in VM group (all P<0.01),and VM in the same subset of clinical-pathologic parameters including Nevin stage,negative liver metastasis and serosal invasion,negative and positive lymph node metastasis (all P<0.05).VEGF was associated with liver metastasis,degree of differentiation in VM group (all P<0.01),and VM in the same subset of clinicopathologic parameters including Nevin stage,negative liver metastasis and moderate degree of differentiation (all P<0.01).Multivariate analysis confirmed that VM,Nevin stage and HIF-1α were independent factors for GBC patients.Conclusions VM exists in human GBC.HIF-1α and VEGF expression is significantly associated with VM and advanced stage in GBC patients.VM and HIF-1α is independent factors for GBC patients.

Cite this article

Sun Wei , Fan Yue-Zu , Zhang Wen-Zhong , Ge Chun-Yan . Vasculogenic mimicry,HIF-1α and VEGF expression in gallbladder carcinoma:association with clinical outcomes and prognostic implications[J]. Surgical Research and New Technique, 2012 , 1(1) : 27 -41 . DOI: 10.3969/j.issn.2095-378X.2012.01.007

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