Objective To investigate the clinical effect of internet-based neuroplasticity learning on the establishment of three-order stereopsis after primary exotropia surgery in children.Methods A total of 62 patients were divided into two groups using internet-based neuroplasticity learning and virtual reality database system: training group (31 cases) and control group (31 cases, i.e.non-training group).All the patients were examined for three-order stereopsis by using the “computer platform-based visual perception examination system” and for stereoscopic recovery half a year after the operation.Results There was no significant difference in three-order stereopsis between the two groups of patients one week after the operation (P>0.05).The children with establishment of the first- and second-order stereopsis in the training group was significantly more than the chilren in the control group (P<0.05), while the children without establishment of three-order stereopsis in the control group was significantly more than the chilren in the training group (P<0.05).Conclusion The neuroplasticity learning based on the internet is helpful to the establishment of three-order stereopsis in children after exotropia surgery, but the stereopsis of the patients who do not use the approach has no obvious improvement.