Objective To analyze the correlation of operating room nursing management with nosocomial infection levels.Methods A total of 405 patients treated from March 2017 to June 2018 in our operating room were selected.Patients were assigned to infection group (n=204) and non-infection group (n=201) according to the nosocomial infection conditions.The correlation of nosocomial infection control levels with operating room nursing management was analyzed.Results The operation time, consecutive operation, incision types, preoperative application of antibiotics, and invasive surgery between the infection group and the non-infection group were statistically different (P<0.05).According to the logistic regression equation, the influencing factors of nosocomial infection included the operation time, consecutive operation, incision types, preoperative application of antibiotics, and invasive surgery.The nosocomial infection was positively correlated with operation time, consecutive operation, Type Ⅱ incision, preoperative application of antibiotics, and invasive surgery (P<0.05).Conclusion The nosocomial infection control levels have a close correlation with the operating room nursing management.Therefore, the medical staff shall strive to summarize the operation experiences, optimize the nursing mode, and improve the nursing management so as to reduce the incidence rate of nosocomial infection.