Objective To understand the distribution and antimicrobial resistance of clinical bacteria,and provide scientific evidence for clinically reasonable use of antibiotics.Methods VITEK-2 Compact automated microbial identification system was used to identify clinical strains.WHONET 5.6 software was used to analyze 3 302 pathogenic bacteria isolated from clinical samples.Results A total of 3 302 strains of pathegenic bacteria were isolated.Of them,the prevalence of gram-negative bacteria was 73% (2 417/3 302) and that of gram-positive bacteria was 27% (885/3 302).The top five isolates were Escherichia coli (24%),Klebsiella pneumoniae (18%),Pseudomonas aeruginosa (10%),Staphylococcus aureus (9%),and Acinetobacter baumannii (9%).For Gram-negative bacteria,ESBLs-producing strains accounted for 50% in Escherichia coli,16.2% in Klebsiella,and 30.2% in Proteus mirabilis.The carbapenem-resistace of Acinetobacter baumannii and Klebsiella pneumonia was serious.For gram-positive bacteria,the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) was 48.2%.The resistance rate of MRSA was higher than that of methicillin-sensitive Staphylococcus aureus (MSSA).Conclusion The bacterial drug-resistance is still a serious issue in clinical setting.Thus,more attention should be paid to multi-drug resistant pathogenic bacteria.Surveillance of bacterial resistance is of great significance to clinical infection control and rational drug use.