Objective To observe and evaluate the efficacy and economic advantages of medical collagen sponge combined with batroxobin in the treatment of mixed hemorrhoids. Methods A total of 120 patients with grade Ⅲ and Ⅳ mixed hemorrhoids were randomly divided into three groups： experimental group, control group, and blank group, with 40 cases in each group. After external dissection and internal ligation, the experimental group was treated with medical collagen sponge combined with batroxobin for hemostasis, the control group with absorbable hemostatic membrane, and the blank group with ordinary gauze. The hemostatic effect, 24 h blood loss amount, postoperative infection, stool bleeding time, wound healing time, and patients' satisfaction were recorded and analyzed. Results The effective rates of the experimental group and the control group were both 100%, and that of the blank group was 75%. The 24 h blood loss amount, postoperative infection incidence, stool bleeding time, and wound healing time of the experimental group were lower than those of the control group and the blank group（P>0.05）, and the satisfaction score was significantly higher（P<0.01）. No postoperative infection occurred in the three groups. Conclusion Medical collagen sponge combined with batroxobin has a satisfying therapeutic effect and economic advantages, which is worthy of promotion.