Objective To analyze the effects of artificial membrane rupture using clamping needle, curved vessel forceps, and sterile cotton swabs during delivery. Methods A total of 91 pregnant women treated in our hospital from September 2019 to August 2021 were divided into three groups according to the random number table method： 30 cases in group A were ruptured with clamping needle, 30 cases in group B with curved vessel forceps, and 31 cases in group C with sterile cotton swabs. The success rate of membrane rupture, the injury of mother and baby during operation, the degree of pain, and the occurrence of occupational exposure of medical staff in the three groups were observed. Results There was no significant difference in the success rate of membrane rupture among the three groups （P>0.05）. There were no significant differences in maternal injury rate and fetal injury rate among the three groups （P>0.05）. There was no significant difference in the degree of pain between group A and group B （P>0.05）, and the degree of pain in group C was lighter than those in group A and group B （P<0.05）. There was no significant difference in the incidence rate of occupational exposure between group A and group B （P>0.05）, and the incidence rate of occupational exposure of medical staff in group C was lower than those in group A and group B （P<0.05）. Conclusion The three membrane breaking methods using clamping needle, tissue forceps, and sterile cotton swabs can avoid the injury of mother and baby during operation and improve the success rate of membrane breaking, but sterile cotton swabs show more significant effects in reducing the degree of pain and the incidence of occupational exposure of medical staff.