Osteosarcoma is the most common primary bone tumor in children and adolescents, with a high degree of malignancy and a poor prognosis. The prevailing treatment paradigm continues to be based on a multimodality therapy of neoadjuvant chemotherapy-surgery-adjuvant chemotherapy, but the survival benefit provided by chemotherapy has remained relatively constant over the past 40 years. Ferroptosis, as a newly discovered form of cell death, has seen rapid research progress over the past decade, and targets for tumor treatment are constantly discovered. Thus, this article commenced with the proposal, developmental nodes, and underlying mechanisms of ferroptosis, then summarized the research results on ferroptosis in osteosarcoma, anticipated its developmental prospects, and highlighted the most urgent things in this field.

[Abstract] Objective To enhance the application value of CT in diagnosis of acute appendicitis. Methods CT findings of 87 cases with acute appendicitis confirmed by surgery and pathology were retrospectively analyzed. Results There were 87 cases of acute appendicitis，45 cases of simple appendicitis，28 cases of suppurative appendicitis，7 cases of gangrenous appendicitis，7 cases of appendiceal abscess. CT findings of 5 cases of simple appendicitis were negative. CT showed swelling of the appendix，edema of the appendix wall and the abscess of the appendix.Conclusion CT imaging is of great value in the diagnosis of acute appendicitis.

[Abstract] Objective To explore the CT findings and differential diagnosis of gastric wall thickening.Methods The CT findings of 82 cases of gastric lesions confirmed by operation and pathology were analyzed retrospectively, compared with normal gastric wall thickness. And to the extent of gastric wall thickness, morphology to identify the characteristics of the CT findings.Results Of the 82 cases 45 were with gastric carcinoma ,17with gastric lymphoma ,12 with gastric stromal tumor ,8 with gastritis. 82 cases with gastric wall thickening were divided into seven groups:group 1 banding type (30), group 2 mass type (14), group3 nodular type (5) ,group 4 ulcerative type (10) , group 5 irregular type (8) , group 6 coral type (0), group 7 mixed type (15).Conclusion CT findings of gastric wall thickening has important value in diagnosis of gastric lesions. With the extent of gastric wall thickening, morphology can be improved imaging diagnosis and differential diagnosis of gastric lesions.

Peroneal tendons dislocation refers to slip the external ankle peroneal tendons in the rear of the fibula groove,inducing a series of symptoms such as instability and pain in the external ankle.There are variously different surgical methods of treating the dislocation of peroneal tendons,each with distinct characteristics.Operation styles mostly consist of soft tissue repair,fibular groove deepening,fibular osteotomy,tissue graft,peroneal tendons rerouting and using of endoscopy in treatment of dislocation.The method of treating peroneal tendons dislocation depend on injury mechanism and patients individual condition for achieving high healing rate,less complications and rapid recovery.

The adult corneal endothelial cells (CEC) could not take self-renewal.The circumjacent cells enlarge and migrate to fill the vacancy after trauma.A normal endothelial cellular density and morphology are pivotal to maintain a normal corneal structure,transparency and refractive index.Traumas,surgeries and some metabolic problems may lead to irreversible results.In this review,the CEC morphological parameters,CEC function and redistribution in a variety of the situation were comprehensively reviewed.Hope it would provide a reference for the treatment of ocular diseases and the judgment of surgical prognosis.

In recent years the application of thermal ablation （TA） in the treatment of benign and malignant thyroid tumors has drawn wide attention. Many studies have shown that compared with traditional surgery, TA is a minimally invasive and cosmetic surgery. In particular, the safety and effectiveness of TA in the treatment of benign thyroid tumors have been generally proven, but are still controversial in the treatment of papillary thyroid microcarcinoma. This article reviewed related literature, and focused on the principles of different thermal ablation techniques, indications and contraindications for the treatment of benign and malignant thyroid tumors, the safety and effectiveness of TA, and the advantages and disadvantages of TA compared with traditional surgery.

This review summarized diagnosis, etiology, risk factors, and harm of the elderly female stress urinary incontinence, expounded the types of stress urinary incontinence among elderly women, treated by non-surgical treatment, and focused on research hot spots in recent years, including lifestyle intervention, voiding habits training, pelvic floor reconstruction rehabilitation, biofeedback and electrical stimulation therapy, and pelvic floor rehabilitation combined with Chinese medicine treatment. In particular, physical therapy and internet plus nursing benefit treatment of urinary incontinence are new hot spots recently. The paper also analyzed the weaknesses and limitations of pelvic floor rehabilitation, the most effective method at present. Medical personnel are required to formulate a practical, simple, and effective treatment.

Objective To study the clinical effect of elastic thread ligation on stage III mixed hemorrhoids. Methods Eighty patients with stage III mixed hemorrhoids were divided into a study group （n=40） and a control group （n=40） randomly. The study group was treated with elastic thread ligation, while the control group was treated with tissue-selecting therapystapler （TST）. Operation time, blood loss volume, postoperative complications, healing time, recurrence rate, and other indicators were observed. Results In the study group： operation time, （18.0±3.5） min; intraoperative blood loss volume, （5.0±1.0） mL; postoperative hemorrhage, 0 case （0）; anal pendant expansion, 2.5% （1/40）; healing time, （18.2±2.5） d. In the control group： operation time, （25.0±7.0） min; intraopereative blood loss volume, （25.0±6.5） mL; postoperative hemorrhage, 5% （2/40）; anal pendant expansion, 15% （6/40）; healing time, （23.8±3.9） d. The study group showed better performance in terms of operation time, blood loss volume, postoperative complications, and healing time than the control group （P<0.05）. Conclusion Elastic thread ligation treatment has advantages in reduced operation time, bleeding amount, complications, and healing time, so it is worthy of clinical promotion.

Radial artery access for coronary angiography and interventions has the advantages of minor invasion, less hemostasis time, improved patient comfort and shorter recovery time, which has been widely adopted in clinical practice. But due to the interventional puncture damage caused by the small size and the various anatomic variants of the radial artery, artery associated complication incidence rate is very high. The purpose of this article is to review the current status and progress of wrist nursing in patients undergoing coronary intervention from the transradial approach.

Electrosurgery enquipments include high-frequency electric knife, argon plasma coagulation, and bipolar vessel-sealing devices. This article introduced the principles and advances of electrosurgery equipments for better understanding and proper use.

Objective To investigate the feasibility of catheterization with a super smooth interventional guidewire combined with a metal catheter and a conventional catheter for male patients who failed conventional catheterization. Methods A total of 205 male patients who failed conventional catheterization were treated with a super smooth interventional guidewire combined with a metal catheter and a conventional catheter. Results Of the 205 patients, 202 had successful catheterization and 3 failed. The success rate of primary catheterization was 98.53%, and the failure rate was 2.47%. Conclusion The use of super smooth interventional guidewire combined with metal catheter for catheterization is a simple, safe, and highly successful method, which is worthy of promotion in clinical practice.

Rotator cuff tear is a common shoulder disease, especially among athletes and middle-aged and elderly individuals. With the accelerating trend of aging population, the number of patients with rotator cuff tears is increasing. For massive rotator cuff tears, there is a high risk of re-tear after surgery and poor prognosis, which poses a significant clinical challenge. In recent years, surgical techniques for massive rotator cuff tears have continued to innovate, including superior capsule reconstruction, subacromial balloon spacer implantation, tendon transfer, and reverse shoulder arthroplasty. This article summarized the biomechanical effects of massive rotator cuff tears on the shoulder joint and focused on the latest treatment advancements, aiming to provide new reference for clinical treatment.

Objective：To study the influence and mechanism of bufalin on apoptosis and calcium metabolism in human colorectal cancer cell HCT 8. Methods：We used the method of CCK-8 to detect the proliferation of HCT 8 treated with bufalin of different concentrations at 24 hours or the same one at different time. And then , the apoptosis of the cell with different concentrations was detected by Annexin V / PI double staining apoptosis kit. Finally, we investigated the effect of different concentrations of bufalin on intracellular Ca2 + by using Ca2+ fluorescence probe Fluo-3 AM to mark its intracellular Ca2+. Results：The inhibition of human colorectal cancer cell HCT 8 with bufalin showed dose and time dependent manner obviously by the method of CCK-8. The result of apoptosis with flow cytometry illustrated that bufalin can induce apoptosis in the cell HCT 8, and represented a dose-dependent manner. According to the results of the intracellular Ca2+ labeled by calcium fluorescent probe calcium, there was a higher concentration of intracellular calcium when the cell treated at a higher concentration of bufalin. Conclusion：Bufalin can induce apoptosis in the cell of HCT 8, and it may be related to the metabolism of intracellular Ca2+.

In terms of distribution of blood supply to the femoral head and neck (FHN),schematic diagrams or planar graphs are expressed in all classical works and textbooks of anatomy and orthopedics.An exact presentation of intra-and extraosseous blood supply to FHN can not be provided which leads to poor understanding of 3D vasculature.On one hand,it is of disadvantage for new learners to know the real circulation to FNN; on the other hand,it makes against reasonable judgment of the severity of local vessels and logical treatment of femoral neck fractures for orthopedic surgeons.To achieve the accurate 3D vasculature networks of FHN through contemporary techniques of radiography and computer image processing.The computer-aided technique of virtual reality is employed for clinical cases of various femoral neck fractures,and further combined with radiographic and histopathological methods to focus on the damaging effect of various femoral neck fractures on the blood supply to the femoral head,and designing individual and optimal treatment protocols for different femoral neck fractures.

Postpartum stress urinary incontinence is an involuntary urine outflow through urethra in women with postpartum abdominal pressure increase, which seriously affects women's physical and mental health and quality of life. This paper summarized and analyzed the research progress on postpartum stress urinary incontinence treated by traditional Chinese medicine from the aspects of etiology and pathogenesis, traditional Chinese materia medica treatment, acupuncture treatment, and other external treatment. Traditional Chinese medicine combined with rehabilitation has a significant effect on postpartum stress urinary incontinence, but there are still some shortages in current research to be improved.

Objective To investigate the videourodynamic study(VUDS) characteristics of various complex lower urinary tract voiding dysfunction and the diagnostic value of videourodynamic examination.Methods From May 2009 to June 2012,78 patients with complex lower urinary tract voiding dysfunction were included in our study.There were 38 males and 40 females,aged 19-75(mean,47) years.All patients had chief complaints of various lower urinary tract symptoms(LUTS),accompanying anatomical abnormalities or other chronic nervous system diseases history,or had a history of failed surgery planning for further surgery treatments.All patients underwent thorough VUDS(uroflowmetry,cystometrogram,pressure-flow study and urethral pressure profile).Results 4 males were removed who couldn’t be intubated the bladder pressure catheter.The remaining 74 cases underwent complete VUDS.8 cases were neurogenic bladder,including DU (3 cases),DO (2 cases),bladder low compliance with uronephrosis(2 cases) and DESD (1 cases).59 cases were non-neurogenic voding dysfunction,including BOO (29 cases),DU (13cases,with 5 female patients who couldn’t void voluntarily in PFS),DO (3 cases),real urinary incontinence(4 cases),stress urinary incontinence after prostatectomy(10 cases).BOO were classified into BPO (3 cases),POP (5 case),bladder neck obstruction (13 cases),posterior urethral obstruction (3 cases) and dysfunctional voiding(5 cases).7 cases were diagnosed normal in VUDS.8 cases changed their previous clinic diagnosis after VUDS.Conclusion Videourodynamic examination provides precise evidence of diagnosis for complex lower urinary tract voiding dysfunction.It can evaluate anatomic abnormalities sites and the function of detrusor/urethral sphincter.It also provides preliminary classification of lower urinary tract diseases and reliable references for the following treatments.

Objective To explore safety control measures and procedures for operating room checklist management during the outbreak of coronavirus disease 2019 (COVID-19), and to ensure the quality of surgical safety and nursing care not affected by the epidemic. Methods Based on the COVID-19 diagnosis and treatment plan, the COVID-19 prevention and control plan, and technical guidelines on the prevention and control of COVID-19 in medical institutions, nursing care tasks in operating rooms were detailed and quantified, and various checklists were formulated to define and improve surgical safety prevention and control measures and procedures in operating rooms. Results During the epidemic, surgeries were succefully performed according to the checklist items, and no safty problems of surgical nursing were found. The checklists identified the respective responsibilities of nurses, improved the working system and workflow during the epidemic, and ensured sufficient supplies of surgical protection materials. Conclusion During the COVID-19 epidemic, checklist management can effectively adjust the nursing measures in operating rooms and optimize workflow. While ensuring surgical operations, it can effectively implement epidemic prevention and control, and improve the work efficiency and quality in operating rooms compared with previous work.