蟾毒灵对人结肠癌HCT 8细胞凋亡及钙离子代谢影响的初步探讨

外科研究与新技术 ›› 2016, Vol. 5 ›› Issue (4): 225-228.

• 论著 • 下一篇

蟾毒灵对人结肠癌HCT 8细胞凋亡及钙离子代谢影响的初步探讨

石晓静¹,孙剑玥²,邱艳艳²,邹瑜¹,袁玉霞¹,贺雪¹,张祎稀²,唐雪瑶¹,殷佩浩²

1. 上海中医药大学附属普陀医院
2. 上海市普陀区中心医院

收稿日期:2016-09-30 修回日期:2016-10-20 出版日期:2016-12-28 发布日期:2017-02-17
通讯作者: 邱艳艳 E-mail:362071162@qq.com
基金资助:
国家自然科学基金;海市科委2016年度医学引导类（中、西医）科技支撑项目

The preliminary study of apoptosis and calcium metabolism in human colorectal cancer cells HCT 8 treated with Bufalin

Received:2016-09-30 Revised:2016-10-20 Online:2016-12-28 Published:2017-02-17

摘要/Abstract

摘要： 目的：初步探讨蟾毒灵对人肠癌HCT8细胞凋亡及钙离子代谢的影响及其机制。方法：采用细胞活性CCK-8法检测不同浓度的蟾毒灵以及相同浓度不同时间孵育后其对人肠癌HCT8细胞增殖的影响；运用Annexin V/PI双染凋亡试剂盒检测不同浓度的蟾毒灵对细胞凋亡的影响情况；采用Ca2+荧光探针Fluo-3 AM孵育人结肠癌HCT8细胞并标记其细胞内的Ca2+，检测不同浓度的蟾毒灵对细胞内Ca2+的影响。结果：CCK-8法结果显示，蟾毒灵对人结肠癌细胞的抑制作用呈明显的剂量以及时间依赖性；流式凋亡结果表明，蟾毒灵能诱导HCT8细胞发生凋亡，且呈一个剂量依赖性；根据Ca2+荧光探针标记胞内钙离子的结果显示，越高浓度的蟾毒灵作用下，细胞内的钙离子浓度越高。结论：蟾毒灵能诱导人结肠癌HCT8细胞凋亡，可能与细胞内的Ca2+代谢相关。

关键词: 结肠癌, 蟾毒灵, 凋亡, 钙离子

Abstract: Objective：To study the influence and mechanism of bufalin on apoptosis and calcium metabolism in human colorectal cancer cell HCT 8. Methods：We used the method of CCK-8 to detect the proliferation of HCT 8 treated with bufalin of different concentrations at 24 hours or the same one at different time. And then , the apoptosis of the cell with different concentrations was detected by Annexin V / PI double staining apoptosis kit. Finally, we investigated the effect of different concentrations of bufalin on intracellular Ca2 + by using Ca2+ fluorescence probe Fluo-3 AM to mark its intracellular Ca2+. Results：The inhibition of human colorectal cancer cell HCT 8 with bufalin showed dose and time dependent manner obviously by the method of CCK-8. The result of apoptosis with flow cytometry illustrated that bufalin can induce apoptosis in the cell HCT 8, and represented a dose-dependent manner. According to the results of the intracellular Ca2+ labeled by calcium fluorescent probe calcium, there was a higher concentration of intracellular calcium when the cell treated at a higher concentration of bufalin. Conclusion：Bufalin can induce apoptosis in the cell of HCT 8, and it may be related to the metabolism of intracellular Ca2+.

Key words: Colorectal cancer, Bufalin, Apoptosis, Ca2+

石晓静孙剑玥邱艳艳邹瑜袁玉霞贺雪张祎稀唐雪瑶殷佩浩. 蟾毒灵对人结肠癌HCT 8细胞凋亡及钙离子代谢影响的初步探讨[J]. 外科研究与新技术, 2016, 5(4): 225-228.

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蟾毒灵对人结肠癌HCT 8细胞凋亡及钙离子代谢影响的初步探讨

The preliminary study of apoptosis and calcium metabolism in human colorectal cancer cells HCT 8 treated with Bufalin

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