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外科研究与新技术（中英文）

外科研究与新技术 ›› 2017, Vol. 6 ›› Issue (2): 87-90.doi: 10.3969/j.issn.2095-378X.2017.02.005

• 论著 • 上一篇    下一篇

护肝解纤汤对肝纤维化相关因子的影响

白珊珊1, 王彦双2, 张晓燕3, 何雅军3   

  1. 1.广州市海珠区疾病预防控制中心,广州 510288;
    2.海南医学院第二附属医院检验科,海口 570100;
    3.暨南大学医学院附属广州市红十字会医院,广州 510220
  • 收稿日期:2017-04-19 发布日期:2017-07-21
  • 通讯作者: 何雅军,电子信箱:heyajun0801@163.com
  • 作者简介:白珊珊(1988—),女,硕士研究生,检验技师,从事临床检验诊断学

The effect of huganjiexian decoction on related factors in liver fibrosis

BAI Shanshan1, WANG Yanshuang2, ZHANG Xiaoyan3, HE Yajun3   

  1. 1.Haizhu District Center for Disease Prevention and Control,Guangzhou 510288,China;
    2.Laboratory Department,The Second Affiliated Hospital of Hainan Medical University, Haikou 570100,China;
    3.The Fourth Affiliated Hospital of Jinan University,Guangzhou Red Cross Hospital, Guangzhou 510220,China
  • Received:2017-04-19 Published:2017-07-21

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摘要： 目的 观察护肝解纤汤对肝纤维化过程中相关细胞因子结缔组织生长因子(CTGF)、转化生长因子(TGF-β1)、血小板衍生生长因子(PDGF)的影响及其可能机制。方法 以四氯化碳腹腔注射8周建立大鼠肝纤维化模型。实验分对照组、模型组、小柴胡汤组和护肝解纤汤组。护肝解纤汤组于大鼠肝纤维化模型鼠第9周起分别予高、中、低剂量护肝解纤汤(处方中姜黄分别为低剂量组6 g,中剂量组9 g,高剂量组15 g,其他成分一致)。免疫组化染色观察肝组织细胞因子CTGF、TGF-β1、PDGF在各组中表达。结果 与模型组比较,高、中剂量护肝解纤汤组肝组织CTGF、PDGF表达显著减少(P<0.05);TGF-β1表达只有高剂量护肝解纤汤组显著降低(P<0.05)。结论 护肝解纤汤组相关促纤维化细胞因子CTGF、TGF-β1、PDGF表达减少,可能是其影响肝纤维化的重要机制。

关键词: 护肝解纤汤, 肝纤维化, 细胞因子, 大鼠

Abstract: Objective To observe the effect and possible mechanism of Huganjiexian Decoction on liver fibrosis-related cytokines CTGF,TGF-β 1,and PDGF.Methods Liver fibrosisr at models wereestablished by intraperitoneal injection of carbon tetrachloride for eight weeks.The rats were divided into control group,model group,Minor Bupleurum Decoction group,and Huganjiexian Decoction group (administered with high,middle,and low doses of turmeric containing 15,9 and 6 g respectively,from week 9-12).Immunohistochemical staining was used to detect the expression of CTGF,TGF-β1,and PDGF in liver tissues.Results The expression levels of CTGF and PDGF in liver tissues in the high and middle dose groups significantly decreased compared to the model group(P<0.05),while the expression level of TGF-β1 decreased significantly only in the high dose group (P<0.05).Conclusion Huganjiexian Decoction could downregulate the expressions of fibrosis-promoting cytokines CTGF,TGF-β1,and PDGF,which might be one of the important mechanism protective effects of against liver fibrosis.

Key words: Huganjiexian Decoction, Hepatic fibrosis, Cytokine, Rats

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