Abstract: Objective To study the possibility and potential mechanisms of epristeride treatment of prostate cancer,and to investigate the effect of epristeride on the growth of DU-145 cells in vitro.Method We used different concentration of epristeride (2.5,5,10,20,40mM) to interfere DU145 cells and used an inverted microscope to observe the cell morphology.We used Sulforhodamine B(SRB) to evaluate the growth curve characterized by the inhibition of in vitro proliferation of DU145 cells caused by epristeride.We applied flow cytometry to evaluate cell cycle arrest of DU145 induced by epristeride.Results An obvious cell shrinkage and apoptosis was observed three days after the interference of epristeride with the concentration of 40mM and 20mM.Epristeride shows a conspicuously concentration-dependent inhibition of in vitro proliferation of DU145 cells.Through flow cytometry,we found that epristeride demonstrated a highly Concentration dependent effect of causing cell cycle arrest in DU145 cell line.

Key words: Epristeride, Apoptosis, Cell proliferation, Sulforhodamine B, Cell line, DU145, Prostate cancer