《中国期刊全文数据库》收录期刊
《中国核心期刊（遴选）数据库》收录期刊
《中文科技期刊数据库》收录期刊
外科研究与新技术（中英文）

外科研究与新技术 ›› 2014, Vol. 3 ›› Issue (3): 169-173,179.

• 论著 • 上一篇    下一篇

The Cardiac Protection Effects of A Single High Loading Dose of Statins Therapy before Coronary Artery Bypass Grafting on Cardiac Specific miRNAs Regulation

张杨杨1,王俊1,李芝1,翟文斌2,陈亦江1   

  1. 1. 南京医科大学第一附属医院，心胸外科
    2. 南京医科大学第一附属临床医学院
  • 收稿日期:2014-10-08 修回日期:2014-10-09 出版日期:2014-09-28 发布日期:2014-12-04
  • 通讯作者: 陈亦江 E-mail:YJChen@njmu.edu.cn
  • 基金资助:
    国家自然科学基金;中华医学会胸心血管外科分会厄尔巴肯奖学金

The Cardiac Protection Effects of A Single High Loading Dose of Statins Therapy before Coronary Artery Bypass Grafting on Cardiac Specific miRNAs Regulation

  • Received:2014-10-08 Revised:2014-10-09 Online:2014-09-28 Published:2014-12-04

PDF (PC)

245

摘要： OBJECTIVE: To explore the cardiac protection effects of a single high loading dose of statins therapy before coronary artery bypass grafting (CABG) on cardiac specific miRNAs (miRNA-1, miRNA-133 miRNA-208 and miRNA-499). METHODS: Patients undergoing CABG were divided into two groups randomly 12 hours before cardiac operation: Group A, receiving standard-dose of 20mg atorvastatin; Group B, receiving single-dose of 80mg atorvastatin. 5 right atriums from group A and 3 from group B were achieved during the operation. Accompanied with 4 right atriums in healthy population as group C, the gene expression on cardiac specific miRNAs (miRNA-1, miRNA-133, miRNA-208 and miRNA-499) of the three groups were examined by a stem-loop quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). RESULTS: The expressions of cardiac specific miRNAs including miRNA-1, miRNA-133a, miRNA-133b, miRNA-208a, miRNA-208b, miRNA-499a-3p, miRNA-499a-5p and miRNA-499b-3p in group A, B and C were not significant changed. Strikingly, miRNA-499b-5p was significantly increased in group B comparing to both healthy group A(p=0.005) and group C (p=0.010). CONCLUSINS: The cardiac protection effect of a single high loading dose of statins therapy before CABG maybe related to elevated miRNA-499b-5p.

Abstract: OBJECTIVE: To explore the cardiac protection effects of a single high loading dose of statins therapy before coronary artery bypass grafting (CABG) on cardiac specific miRNAs (miRNA-1, miRNA-133 miRNA-208 and miRNA-499). METHODS: Patients undergoing CABG were divided into two groups randomly 12 hours before cardiac operation: Group A, receiving standard-dose of 20mg atorvastatin; Group B, receiving single-dose of 80mg atorvastatin. 5 right atriums from group A and 3 from group B were achieved during the operation. Accompanied with 4 right atriums in healthy population as group C, the gene expression on cardiac specific miRNAs (miRNA-1, miRNA-133, miRNA-208 and miRNA-499) of the three groups were examined by a stem-loop quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). RESULTS: The expressions of cardiac specific miRNAs including miRNA-1, miRNA-133a, miRNA-133b, miRNA-208a, miRNA-208b, miRNA-499a-3p, miRNA-499a-5p and miRNA-499b-3p in group A, B and C were not significant changed. Strikingly, miRNA-499b-5p was significantly increased in group B comparing to both healthy group A(p=0.005) and group C (p=0.010). CONCLUSINS: The cardiac protection effect of a single high loading dose of statins therapy before CABG maybe related to elevated miRNA-499b-5p.