胆囊癌血管生成拟态,缺氧诱导因子1α和血管内皮生长因子表达及其与临床预后的关系

Abstract

Abstract: Purpose To explore whether VM exists in human gallbladder carcinoma (GBC) and VM’ correlation with HIF-1α,VEGF and prognosis of GBC patients.Methods HE and CD31-PAS staining were used to observe VM in GBC.Immunohistochemistry (IHC),computer-assisted image and receiver operating characteristic curve were used to analyze expression of HIF-1α and VEGF in GBC,IHC quantitative analysis and selection of the cut-off score for high or low IHC reactivity.Correlation of HIF-1α and VEGF expression with clinicopathologic features and prognosis of GBC patients with VM and non-VM was evaluated simultaneously.Results VM was observed in GBC (21.35%) and associated with adenocarcinoma and liver metastasis (both P<0.01).Over-expression of HIF-1α and reduced-expression of VEGF were observed in GBC patients with VM (both P<0.01).HIF-1α was correlated with serosal invasion,degree of differentiation in VM group (all P<0.01),and VM in the same subset of clinical-pathologic parameters including Nevin stage,negative liver metastasis and serosal invasion,negative and positive lymph node metastasis (all P<0.05).VEGF was associated with liver metastasis,degree of differentiation in VM group (all P<0.01),and VM in the same subset of clinicopathologic parameters including Nevin stage,negative liver metastasis and moderate degree of differentiation (all P<0.01).Multivariate analysis confirmed that VM,Nevin stage and HIF-1α were independent factors for GBC patients.Conclusions VM exists in human GBC.HIF-1α and VEGF expression is significantly associated with VM and advanced stage in GBC patients.VM and HIF-1α is independent factors for GBC patients.

Key words: Vasculogenic mimicry, Hypoxia inducible factor-1α, Vascular endothelial growth factor, Gallbladder neoplasm, Prognosis

CLC Number:

R575.6

Sun Wei, Fan Yue-Zu, Zhang Wen-Zhong, Ge Chun-Yan. Vasculogenic mimicry,HIF-1α and VEGF expression in gallbladder carcinoma:association with clinical outcomes and prognostic implications[J]. 外科研究与新技术, 2012, 1(1): 27-41.

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Vasculogenic mimicry,HIF-1α and VEGF expression in gallbladder carcinoma:association with clinical outcomes and prognostic implications

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