Abstract: [Abstract] Objective: To investigate the distribution and antibacterial susceptibility of bacterias in bile and antibacterial selection of patients with biliary tract diseases. Methods: The results of bacterial cultivation and drug-sensitivity tests of bile samples from 289 patients in general surgical ward in our hospital and these patients' consumption of antibacterials from January 2011 to September 2016 were collected and analyzed retrospectively. Results: The positive rate of tests was 39.5%. Gram-negative bacterias accounted for 71.9% and gram-positive bacterias accounted for 28.1%. The top 6 pathogens were Escherichia Coli (21.9%), Klebsiella Pneumoniae (13.3%), Pseudomonas Aeruginosa (10.1%), Enterococcus Faecalis (9.4%), Enterobacter Cloacae (7%). Escherichia coli had high rate of resistance to most antibacterials. Enterococcus faecalis had low rate of resistance to vancomycin, linezolid, and penicillin G. The top 5 antibacterials sorted by DDDs were ornidazole, cefuroxime, Cefmetazole, cefepime, latamoxef. Conclusion: The antibacterial seletion wasn't fully justified by the results of bacterial cultivation and drug-sensitivity tests. Doctors should think highly of microbiological examination of bile and select antibacterials according to the results.

Key words: [Key words] bile, antibacterial susceptibility, antibacterials