携带TAp63的双靶向溶瘤腺病毒抑制结肠癌干细胞生长

doi:10.3969/j.issn.2095-378X.2018.02.001

Surgical Research and New Technique ›› 2018, Vol. 7 ›› Issue (2): 77-82.doi: 10.3969/j.issn.2095-378X.2018.02.001

• Original article • Next Articles

Dual-regulated oncolytic adenoviruses carrying TAp63 geneinhibit the growth of colon cancer stem cells

LUO Qifeng^1,2, DU Min³, DAI Zhenling⁴, FU Zhengwei², ZHANG Zhenyu², Shiva BASNET², GE Haiyan^1,2

1.Department of General Surgery, Pinghu Second People’s Hospital, Pinghu 314200, China;
2.Department of General Surgery, Shanghai East Hospital, Tongji University, Shanghai 200120, China;
3.Department of Burn and Plastic Surgery, Qilu Hospital, Shandong University, Jinan 250012, China;
4.Department of General Surgery, Gongli Hospital, Pudong New Area, Shanghai 200135, China

Received:2018-04-13 Online:2018-06-28 Published:2019-12-10

Abstract

Abstract: Objective To construct dual-regulated oncolytic adenoviruses carrying TAp63 gene and detect their antitumor effect on colon cancer stem cells.Methods Colon cancer stem cells were screened by flow cytometry.New recombinant adenoviruses were constructed according to the Adeasy system.The viral DNA acted as a template for PCR, then agarose gel electrophoresis assay was applied to confirm the viral DNA.CCK8 assay and cell apoptosis assay were used to detect the effects of recombinant adenoviruses on the proliferation of colon cancer stem cells and human normal liver cells, as well as on the apoptosis of colon cancer stem cells.Results The proportion of CD133/CD44 double positive cells was (42.47±0.81)% in HCT116 cells (P<0.05).The recombinant adenoviruses Arsz-TAp63 were successfully constructed without wild-type adenovirus contamination, and were shown to inhibit the proliferation of colon cancer stem cells HCT116 ^CD133/CD44, but not human normal liver cells L-02, and promote the apoptosis of HCT116 ^CD133/CD44cells.Conclusion The recombinant adenoviruses Arsz-TAp63 selectively inhibit the proliferation and induce the apoptosis of HCT116 ^CD133/CD44 cells.

Key words: Colon cancer, Stem cell, Oncolytic adenovirus

CLC Number:

R735.3

LUO Qifeng, DU Min, DAI Zhenling, FU Zhengwei, ZHANG Zhenyu, Shiva BASNET, GE Haiyan. Dual-regulated oncolytic adenoviruses carrying TAp63 geneinhibit the growth of colon cancer stem cells[J]. Surgical Research and New Technique, 2018, 7(2): 77-82.

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Dual-regulated oncolytic adenoviruses carrying TAp63 geneinhibit the growth of colon cancer stem cells

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