ATM-/-小鼠Sertoli细胞对于双链DNA损伤修复的初步研究

Abstract

Abstract: [Abstract] Objective To Study the repair of DNA double-strand break(DSB) in ATM-/- mice sertoli cells. Methods 30 4-5wk C57 male mice were randomly divided into ATM-/- mice group and control group,of which were randomly divided into 0.1Gy X-ray irradiation(ionizing irradiation)0 min,45min,2hr,24hr,48hr groups ,immunofluorescence staining of DAPI and γ-H2Ax were done. Results After low-dose irradiation,ATM-/- mice sertoli cells have a lower repair efficiency than wild type mice germ cells. Conclusion Efficiency of DSB repair in ATM-/- mice sertoli cells is lower than that of C57 mice.

Key words: Sertoli cell, ATM, DNA double-strand break, DNA damage。

References

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Study on the DNA Double-Strand break repair of ATM -/- mice sertoli cells

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