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Surgical Research and New Technique ›› 2016, Vol. 5 ›› Issue (2): 96-101.

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Construction and identification of Fn14-shRNA lentiviral vector

  

  • Received:2016-01-18 Revised:2016-01-19 Online:2016-06-28 Published:2016-09-13

Abstract: Objective To construct a Fn14-shRNA lentiviral vector to further study the expression and biological significance of Fn14 in osteosarcoma with animal experiment. Methods Constructing Fn14-shRNA vector by RNA interference technique, viral packaging, co-transfecting cells and indentification. Results Single enzyme detection was positive, sequencing result was consistent with design, Fn14 was downregulated in stably transfected cell lines. Conclusion The Fn14-sh RNA lentivirus recombinant vectors were successfully constructed which laid the foundation for further study of the biological significance of Fn14 in osteosarcoma.

Key words: osteosarcoma, Fibroblast growth factor-inducible14 (Fn14), RNA intervention, lentiviral