Abstract: Objective To prepare oxidized regenerated cellulose/chitosan （ORC-CTS） hemostatic sponges, and study its properties. Methods ORC-CTS hemostatic sponges were prepared and characterized. The hemostatic properties and prognostic effects of ORC-CTS, chitosan, and surgicel were compared by hemostasis test, inflammation test, and toxicity test in vivo. Results The blood loss in the ORC-CTS group was less than that in the control group, the chitosan group, and the surgicel group （all P<0.05）; the hemostasis time in the ORC-CTS group was shorter than that in the control group, the chitosan group, and the surgicel group （all P<0.05）. The degree of inflammation ranked as control group<ORC-CTS group<chitosan group<surgicel group. No significant differences in serum tumor necrosis factor （TNF-α）, isoprostanes, and von Willebrand Factor （vWF） were found between the ORC-CTS group and the control group. Conclusion ORC-CTS hemostatic sponges can reduce blood loss, shorten bleeding time, stimulate mild inflammatory reaction, show good biocompatibility and little toxicity, thus may be an ideal new hemostatic material.

Key words: Oxidized regenerated cellulose, Chitosan, Hemostatic sponge